A study published in Gastroenterology describes a new mitochondrial mediator in the hepatotoxicity induced by paracetamol. Institute of Biomedical Research of Barcelona Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter PARACETAMOL DEPLETES GLUTATHIONE Natural Healing Centre