Could Tylenol be making your child's health worse? Acetaminophen is widely used for pain and fever relief, but research suggests it depletes glutathione, a powerful antioxidant essential for immune function, Highly Selective and Prolonged Depletion of Mitochondrial Glutathione in Astrocytes Markedly Increases Sensitivity to Peroxynitrite Journal of Neuroscience Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Benefits of Glutathione Helps Detox Your Liver And Muc Water for Health