What is glutathione? Know as the most important antioxidant in the body, glutathione is an essential antioxidant manufactured in all cells, but primarily in the liver. It is essential because it Glutathione depletion induces ferroptosis, autophagy, and premature cell senescence in retinal pigment epithelial cells Cell Death & Disease Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter