Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione Single Peptide RegenMD Glutathione Depleting Pro Oxidant as a Selective Anticancer Therapeutic Agent ACS Omega Glutathione, often dubbed the master antioxidant plays a crucial role in detoxification, immune function, and protecting our cells from oxidative stress. Here are some signs that your levels might be low: Persistent